Part:BBa_K3103009:Design

pETse_int_N_r

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

We did an initial screening with the sequencing primers that flank the gene insertion site in the vector backbone. Every plasmid backbone has a _f and_r sequencing primer which is used to sequencing interred genes, and can also be used to PCR the insert up. When screening by PCR we looked for a specific size (size of insert plus flanking sequence). To confirm OUR gene was inserted, we wanted a specific primer which only amplified our gene of interest. This is a lot more diagnostic. We did this by making primers specific to the gene, and using one of the _f or _r primers in conjunction with the sequencing _r and _f primers.

Source

pCS-PET_VB190412